Publication Details

Regulation of gene expression and cocaine reward by CREB and DeltaFosB.

Authors:McClung CA, Nestler EJ
Title:Regulation of gene expression and cocaine reward by CREB and DeltaFosB.
Journal:Nature neuroscience Nov 2003 , Vol 6 , pp. 1208-15
Abstract:DeltaFosB (a truncated form of FosB) and CREB (cAMP response element binding protein) are transcription factors induced in the brain's reward pathways after chronic exposure to drugs of abuse. However, their mechanisms of action and the genes they regulate remain unclear. Using microarray analysis in the nucleus accumbens of inducible transgenic mice, we found that CREB and a dominant-negative CREB have opposite effects on gene expression, as do prolonged expression of DeltaFosB and the activator protein-1 (AP-1) antagonist DeltacJun. However, unlike CREB, short-term and prolonged DeltaFosB induction had opposing effects on gene expression. Gene expression induced by short-term DeltaFosB and by CREB was strikingly similar, and both reduced the rewarding effects of cocaine, whereas prolonged DeltaFosB expression increased drug reward. Gene expression after a short cocaine treatment was more dependent on CREB, whereas gene expression after a longer cocaine treatment became increasingly DeltaFosB dependent. These findings help define the molecular functions of CREB and DeltaFosB and identify clusters of genes that contribute to cocaine addiction.   PUBMED: 14566342
Ontological Annotations:
  • D009714: Nucleus Accumbens (Publication, NCBO Annotator; PubMed MeSH Annotations)
  • D020218: Response Elements (Publication, NCBO Annotator)
  • D020411: Oligonucleotide Array Sequence Analysis (PubMed MeSH Annotations)
  • D016000: Cluster Analysis (PubMed MeSH Annotations)
  • D017579: Domestic Violence (Publication, NCBO Annotator)
  • D000818: Animals (PubMed MeSH Annotations)
  • D012201: Reward (Publication, NCBO Annotator; PubMed MeSH Annotations)
  • D004364: Pharmaceutical Preparations (Publication, NCBO Annotator)
  • D005786: Gene Expression Regulation (PubMed MeSH Annotations; Publication, NCBO Annotator)
  • MP:0002555: addiction (Publication, NCBO Annotator)
  • D010751: Phosphopyruvate Hydratase (PubMed MeSH Annotations)
  • GO:0010468: regulation of gene expression (Publication, NCBO Annotator)
  • D019970: Cocaine-Related Disorders (Publication, NCBO Annotator)
  • D012926: Social Control, Formal (Publication, NCBO Annotator)
  • D002467: Cell Nucleus (Publication, NCBO Annotator)
  • D016739: Behavior, Addictive (Publication, NCBO Annotator; PubMed MeSH Annotations)
  • D020133: Reverse Transcriptase Polymerase Chain Reaction (PubMed MeSH Annotations)
  • D004602: Elements (Publication, NCBO Annotator)
  • D016760: Proto-Oncogene Proteins c-fos (PubMed MeSH Annotations)
  • D003042: Cocaine (PubMed MeSH Annotations; Publication, NCBO Annotator)
  • GO:0005634: nucleus (Publication, NCBO Annotator)
  • D019966: Substance-Related Disorders (Publication, NCBO Annotator)
  • D001426: Bacterial Proteins (PubMed MeSH Annotations)
  • D000779: Anesthetics, Local (PubMed MeSH Annotations)
  • D046228: Microarray Analysis (Publication, NCBO Annotator)
  • D001522: Behavior, Animal (PubMed MeSH Annotations)
  • D004552: Elder Abuse (Publication, NCBO Annotator)
  • D012333: RNA, Messenger (PubMed MeSH Annotations)
  • D013287: Street Drugs (Publication, NCBO Annotator)
  • GO:0005488: binding (Publication, NCBO Annotator)
  • D051379: Mice (PubMed MeSH Annotations; Publication, NCBO Annotator)
  • D016755: Proto-Oncogene Proteins c-jun (PubMed MeSH Annotations)
  • D013812: Therapeutics (Publication, NCBO Annotator)
  • GO:0010467: gene expression (Publication, NCBO Annotator)
  • D003213: Conditioning (Psychology) (PubMed MeSH Annotations)
  • D014157: Transcription Factors (Publication, NCBO Annotator)
  • D008822: Mice, Transgenic (PubMed MeSH Annotations; Publication, NCBO Annotator)
  • D017362: Cyclic AMP Response Element-Binding Protein (PubMed MeSH Annotations)
  • D013995: Time (PubMed MeSH Annotations)
  • D018808: Transcription Factor AP-1 (Publication, NCBO Annotator)
  • MA:0000892: accumbens nucleus (Publication, NCBO Annotator)
  • D017353: Gene Deletion (PubMed MeSH Annotations)
  • D013997: Time Factors (PubMed MeSH Annotations)

32 GeneSets from this Publication:


Select gene sets below using the check boxes, and then add them to a project using the pull down below. You can also create a project using the pull down. To view a set, click on the GeneSetID:GeneSet Description shown in green below.
Expand Tier I Mouse DRG 115 Genes provisional GS86386: Table S1: GENES UPREGULATED BY CREB AFTER 8 WEEKS OVEREXPRESSION VS.CONTROLS AND THE EFFECT OF mCREB [DRG]
Expand Tier I Mouse DRG 106 Genes provisional GS86414: Table S1: GENES UPREGULATED BY CREB AFTER 8 WEEKS OVEREXPRESSION VS.CONTROLS AND THE EFFECT OF mCREB [DRG]
Expand Tier I Mouse DRG 34 Genes provisional GS86424: Table S6: GENES UPREGULATED AFTER 8 WEEKS DELTAFOSB VS.CONTROLS AND THE EFFECT AT 1, 2 AND 4 WEEKS [DRG]
Expand Tier I Mouse DRG 50 Genes provisional GS86458: Table S10: GENES UPREGULATED AFTER 2 WEEKS DELTAFOSB VS.CONTROLS AND THE EFFECT OF CREB, 8 WEEKS DELTAFOSB, AND MCREB [DRG]
Expand Tier I Mouse DRG 116 Genes provisional GS86560: Table S3: GENES UPREGULATED BY CREB AFTER 8 WEEKS OVEREXPRESSION VS.CONTROLS AND THE EFFECT OF CREB AFTER 2 WEEKS [DRG]
Expand Tier I Mouse DRG 48 Genes provisional GS86584: Table S8: GENES UPREGULATED AFTER 8 WEEKS DELTAFOSB VS.CONTROLS AND THE EFFECT OF DELTACJUN [DRG]
Expand Tier I Mouse DRG 49 Genes provisional GS86596: Table S6: GENES UPREGULATED AFTER 8 WEEKS DELTAFOSB VS.CONTROLS AND THE EFFECT AT 1, 2 AND 4 WEEKS [DRG]
Expand Tier I Mouse DRG 39 Genes provisional GS86635: Table S10: GENES UPREGULATED AFTER 2 WEEKS DELTAFOSB VS.CONTROLS AND THE EFFECT OF CREB, 8 WEEKS DELTAFOSB, AND MCREB [DRG]
Expand Tier I Mouse DRG 49 Genes provisional GS86636: Table S6: GENES UPREGULATED AFTER 8 WEEKS DELTAFOSB VS.CONTROLS AND THE EFFECT AT 1, 2 AND 4 WEEKS [DRG]
Expand Tier I Mouse DRG 44 Genes provisional GS86637: Table S7: GENES UPREGULATED AFTER 2 WEEKS DELTAFOSB VS.CONTROLS AND THE EFFECT OF DELTACJUN [DRG]
Expand Tier I Mouse DRG 35 Genes provisional GS86639: Table S4: GENES UPREGULATED BY CREB AFTER 2 WEEKS OVEREXPRESSION VS.CONTROLS AND THE EFFECT OF CREB AFTER 8 WEEKS [DRG]
Expand Tier I Mouse DRG 44 Genes provisional GS86640: Table S10: GENES UPREGULATED AFTER 2 WEEKS DELTAFOSB VS.CONTROLS AND THE EFFECT OF CREB, 8 WEEKS DELTAFOSB, AND MCREB [DRG]
Expand Tier I Mouse DRG 64 Genes provisional GS86692: Table S2: GENES DOWNREGULATED BY CREB AFTER 8 WEEKS OVEREXPRESSION VS.CONTROLS AND THE EFFECT OF mCREB [DRG]
Expand Tier I Mouse DRG 48 Genes provisional GS86703: Table S10: GENES UPREGULATED AFTER 2 WEEKS DELTAFOSB VS.CONTROLS AND THE EFFECT OF CREB, 8 WEEKS DELTAFOSB, AND MCREB [DRG]
Expand Tier I Mouse DRG 30 Genes provisional GS86722: Table S4: GENES UPREGULATED BY CREB AFTER 2 WEEKS OVEREXPRESSION VS.CONTROLS AND THE EFFECT OF CREB AFTER 8 WEEKS [DRG]
Expand Tier I Mouse DRG 50 Genes provisional GS86773: Table S7: GENES UPREGULATED AFTER 2 WEEKS DELTAFOSB VS.CONTROLS AND THE EFFECT OF DELTACJUN [DRG]
Expand Tier I Mouse DRG 101 Genes provisional GS86821: Table S3: GENES UPREGULATED BY CREB AFTER 8 WEEKS OVEREXPRESSION VS.CONTROLS AND THE EFFECT OF CREB AFTER 2 WEEKS [DRG]
Expand Tier I Mouse DRG 50 Genes provisional GS86866: Table S5: GENES UPREGULATED AFTER 2 WEEKS DELTAFOSB VS.CONTROLS AND THE EFFECT AT 1, 4 AND 8 WEEKS [DRG]
Expand Tier I Mouse DRG 50 Genes provisional GS86874: Table S2: GENES DOWNREGULATED BY CREB AFTER 8 WEEKS OVEREXPRESSION VS.CONTROLS AND THE EFFECT OF mCREB [DRG]
Expand Tier I Mouse DRG 109 Genes provisional GS86876: Table S9: GENES UPREGULATED AFTER 8 WEEKS OF CREB AND THE EFFECT OF 2 AND 8 WKS DELTAFOSB AND MCREB [DRG]
Expand Tier I Mouse DRG 44 Genes provisional GS86886: Table S5: GENES UPREGULATED AFTER 2 WEEKS DELTAFOSB VS.CONTROLS AND THE EFFECT AT 1, 4 AND 8 WEEKS [DRG]
Expand Tier I Mouse DRG 39 Genes provisional GS87022: Table S5: GENES UPREGULATED AFTER 2 WEEKS DELTAFOSB VS.CONTROLS AND THE EFFECT AT 1, 4 AND 8 WEEKS [DRG]
Expand Tier I Mouse DRG 115 Genes provisional GS87039: Table S9: GENES UPREGULATED AFTER 8 WEEKS OF CREB AND THE EFFECT OF 2 AND 8 WKS DELTAFOSB AND MCREB [DRG]
Expand Tier I Mouse DRG 106 Genes provisional GS87063: Table S9: GENES UPREGULATED AFTER 8 WEEKS OF CREB AND THE EFFECT OF 2 AND 8 WKS DELTAFOSB AND MCREB [DRG]
Expand Tier I Mouse DRG 91 Genes provisional GS87071: Table S9: GENES UPREGULATED AFTER 8 WEEKS OF CREB AND THE EFFECT OF 2 AND 8 WKS DELTAFOSB AND MCREB [DRG]
Expand Tier I Mouse DRG 54 Genes provisional GS87088: Table S8: GENES UPREGULATED AFTER 8 WEEKS DELTAFOSB VS.CONTROLS AND THE EFFECT OF DELTACJUN [DRG]
Expand Tier I Mouse DRG 45 Genes provisional GS87120: Table S5: GENES UPREGULATED AFTER 2 WEEKS DELTAFOSB VS.CONTROLS AND THE EFFECT AT 1, 4 AND 8 WEEKS [DRG]
Expand Tier I Mouse DRG 54 Genes provisional GS87150: Table S6: GENES UPREGULATED AFTER 8 WEEKS DELTAFOSB VS.CONTROLS AND THE EFFECT AT 1, 2 AND 4 WEEKS [DRG]
Expand Tier I Mouse DRG 39 Genes provisional GS87267: Table S10: GENES UPREGULATED AFTER 2 WEEKS DELTAFOSB VS.CONTROLS AND THE EFFECT OF CREB, 8 WEEKS DELTAFOSB, AND MCREB (Whole Table) [DRG]
Expand Tier I Mouse DRG 39 Genes provisional GS87277: Table S5: GENES UPREGULATED AFTER 2 WEEKS DELTAFOSB VS.CONTROLS AND THE EFFECT AT 1, 4 AND 8 WEEKS (Whole Table) [DRG]
Expand Tier I Mouse DRG 54 Genes provisional GS87284: Table S8: GENES UPREGULATED AFTER 8 WEEKS DELTAFOSB VS.CONTROLS AND THE EFFECT OF DELTACJUN (Whole Table) [DRG]
Expand Tier I Mouse DRG 92 Genes provisional GS87289: Table S9: GENES UPREGULATED AFTER 8 WEEKS OF CREB AND THE EFFECT OF 2 AND 8 WKS DELTAFOSB AND MCREB (Whole Table) [DRG]