Publication Details

Gene expression in human alcoholism: microarray analysis of frontal cortex.

Authors:Lewohl JM, Wang L, Miles MF, Zhang L, Dodd PR, Harris RA
Title:Gene expression in human alcoholism: microarray analysis of frontal cortex.
Journal:Alcoholism, clinical and experimental research Dec 2000 , Vol 24 , pp. 1873-82
Abstract:Changes in brain gene expression are thought to be responsible for the tolerance, dependence, and neurotoxicity produced by chronic alcohol abuse, but there has been no large scale study of gene expression in human alcoholism.RNA was extracted from postmortem samples of superior frontal cortex of alcoholics and nonalcoholics. Relative levels of RNA were determined by array techniques. We used both cDNA and oligonucleotide microarrays to provide coverage of a large number of genes and to allow cross-validation for those genes represented on both types of arrays.Expression levels were determined for over 4000 genes and 163 of these were found to differ by 40% or more between alcoholics and nonalcoholics. Analysis of these changes revealed a selective reprogramming of gene expression in this brain region, particularly for myelin-related genes which were down-regulated in the alcoholic samples. In addition, cell cycle genes and several neuronal genes were changed in expression.These gene expression changes suggest a mechanism for the loss of cerebral white matter in alcoholics as well as alterations that may lead to the neurotoxic actions of ethanol.   PUBMED: 11141048
Ontological Annotations:
  • D001927: Brain Diseases (Publication, NCBO Annotator)
  • D006801: Humans (Publication, NCBO Annotator; PubMed MeSH Annotations)
  • D020411: Oligonucleotide Array Sequence Analysis (PubMed MeSH Annotations; Publication, NCBO Annotator)
  • D017579: Domestic Violence (Publication, NCBO Annotator)
  • D009841: Oligonucleotides (Publication, NCBO Annotator)
  • MA:0000945: cerebral white matter (Publication, NCBO Annotator)
  • D000368: Aged (PubMed MeSH Annotations)
  • D000369: Aged, 80 and over (PubMed MeSH Annotations)
  • MA:0001135: white matter (Publication, NCBO Annotator)
  • D018816: Genes, cdc (Publication, NCBO Annotator)
  • D001921: Brain (Publication, NCBO Annotator)
  • D005190: Family (Publication, NCBO Annotator)
  • D009185: Myelin Proteins (PubMed MeSH Annotations)
  • D002477: Cells (Publication, NCBO Annotator)
  • D002453: Cell Cycle (Publication, NCBO Annotator)
  • GO:0007049: cell cycle (Publication, NCBO Annotator)
  • MA:0000168: brain (Publication, NCBO Annotator)
  • D054022: Cerebrum (Publication, NCBO Annotator)
  • D000438: Alcohols (Publication, NCBO Annotator)
  • D000431: Ethanol (Publication, NCBO Annotator)
  • GO:0005623: cell (Publication, NCBO Annotator)
  • D007854: Lead (Publication, NCBO Annotator)
  • D008875: Middle Aged (PubMed MeSH Annotations)
  • D005625: Frontal Lobe (PubMed MeSH Annotations)
  • D012313: RNA (Publication, NCBO Annotator)
  • D019966: Substance-Related Disorders (Publication, NCBO Annotator)
  • D014930: Women (Publication, NCBO Annotator)
  • D046228: Microarray Analysis (Publication, NCBO Annotator)
  • D020258: Neurotoxicity Syndromes (Publication, NCBO Annotator)
  • D004552: Elder Abuse (Publication, NCBO Annotator)
  • D008722: Methods (Publication, NCBO Annotator)
  • D013618: Tachyphylaxis (Publication, NCBO Annotator)
  • D000303: Adrenal Cortex Diseases (Publication, NCBO Annotator)
  • MA:0000905: frontal cortex (Publication, NCBO Annotator)
  • D000437: Alcoholism (PubMed MeSH Annotations; Publication, NCBO Annotator)
  • GO:0010467: gene expression (Publication, NCBO Annotator)
  • D018076: DNA, Complementary (Publication, NCBO Annotator)
  • D015536: Down-Regulation (PubMed MeSH Annotations)
  • D057229: Alcoholics (Publication, NCBO Annotator)
  • D044465: European Continental Ancestry Group (Publication, NCBO Annotator)

6 GeneSets from this Publication:


Select gene sets below using the check boxes, and then add them to a project using the pull down below. You can also create a project using the pull down. To view a set, click on the GeneSetID:GeneSet Description shown in green below.
Expand Tier III Human 147 Genes GS75605: Frontal cortex gene expression in alcoholic vs nonalcoholic humans 1
Expand Tier V Human 147 Genes GS75606: Frontal cortex gene expression in alcoholic vs nonalcoholic humans 2
Expand Tier I Human DRG 7 Genes provisional GS87255: Table 2: Myelin-Related Expression Data (Whole Table) [DRG]
Expand Tier I Human DRG 9 Genes provisional GS87256: Table 2: Myelin-Related Expression Data (Whole Table) [DRG]
Expand Tier I Human DRG 10 Genes provisional GS87257: Table 2: Myelin-Related Expression Data (Whole Table) [DRG]
Expand Tier I Human DRG 8 Genes provisional GS87258: Table 2: Myelin-Related Expression Data (Whole Table) [DRG]